Enteric Bacterial Pathogens Laboratory - Publications

Primary data articles:

  • Carnell SC, Bowen AJ, Morgan E, Maskell DJ, Wallis TS, Stevens M. (2007) Role in virulence and protective efficacy in pigs of Salmonella enterica serovar Typhimurium secreted components identified by signature-tagged mutagenesis. Microbiology. In press.  
  • Hudson D, Field T, Bowen A, Bispham J, Wolf-Watz H, Stevens MP, Galyov EE. (2007) Inhibition of Salmonella Type III secretion by small-molecule inhibitors. Antimicrob. Agents Chemother. 
  • Dziva F, Mahajan A, Cameron P, Currie C, McKendrick IJ, Wallis TS, Smith DG, Stevens MP. (2007) EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157:H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells. FEMS Microbiol Lett [Epub ahead of print]. 
  • Dziva F, Vlisidou I, Crepin VF, Wallis TS, Frankel G, Stevens MP. (2007) Vaccination of calves with EspA, a key colonisation factor of Escherichia coli O157:H7, induces antigen-specific humoral responses but does not confer protection against intestinal colonisation. Vet Microbiol [Epub ahead of print]. 
  • van Diemen PM, Dziva F, Abu-Median A, Wallis TS, van den Bosch H, Dougan G, Chanter N, Frankel G, Stevens MP. (2007) Subunit vaccines based on intimin and Efa-1 polypeptides induce humoral immunity in cattle but do not protect against intestinal colonisation by enterohaemorrhagic Escherichia coli O157:H7 or O26:H-. Vet Immunol Immunopathol , 116:47-58 . 
  • Girard F, Dziva F, van Diemen P, Phillips AD, Stevens MP, Frankel G. (2007) Adherence of enterohemorrhagic Escherichia coli O157, O26 and O111 strains to bovine intestinal explants ex vivo. Appl Environ Microbiol [Epub ahead of print]. 
  • Ooka T, Vieira MA, Ogura Y, Beutin L, La Ragione R, van Diemen PM, Stevens MP, Aktan I, Cawthraw S, Best A, Hernandes RT, Krause G, Gomes TA, Hayashi T, Frankel G. (2007) Characterization of tccP2 carried by atypical enteropathogenic Escherichia coli. FEMS Microbiol Lett [Epub ahead of print]. 
  • Morgan E, Bowen AJ, Carnell SC, Wallis TS, Stevens MP. (2007) SiiE is secreted by the Salmonella enterica serovar Typhimurium pathogenicity island 4-encoded secretion system and contributes to intestinal colonization in cattle. Infect Immun , 75:1524-33 . 
  • Haque A, Chu K, Easton A, Stevens MP, Galyov EE, Atkins T, Titball R, Bancroft GJ. (2006) A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins. Journal of Infect Dis , 194:1241-8 . 
  • Vlisidou I, Marches O, Dziva F, Mundy R, Frankel G, Stevens MP. (2006) Identification and characterization of EspK, a type III secreted effector protein of enterohaemorrhagic Escherichia coli O157:H7. FEMS Microbiol Lett , 263:32-40 . 
  • Culver F, Dziva F, Cavanagh D, Stevens MP (2006) Poult enteritis and mortality syndrome in turkeys in Great Britain. Vet Re , 159:209-10 . 
  • Chen C, Lyte M, Stevens MP, Vulchanova L, Brown DR. (2006) Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon. Eur J Pharmacol , 539:116-24 . 
  • Boyen F, Pasmans F, Van Immerseel F, Morgan E, Adriaensen C, Hernalsteens JP, Decostere A, Ducatelle R, Haesebrouck F. (2006) Salmonella Typhimurium SPI-1 genes promote intestinal but not tonsillar colonization in pigs. Microbes Infect , 8:2899-907 . 
  • Chattoo JP, Stevens MP, Nair V. (2006) Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis. Journal of Virol Methods , 135:288-91 . 
  • Low AS, Dziva F, Torres AG, Martinez JL, Rosser T, Naylor S, Spears K, Holden N, Mahajan A, Findlay J, Sales J, Smith DG, Low JC, Stevens MP, Gally DL. (2006) Cloning, expression, and characterization of fimbrial operon F9 from enterohemorrhagic Escherichia coli O157:H7. Journal of Infect Immun , 74:2233-44 . 
  • Vlisidou I, Dziva F, La Ragione RM, Best A, Garmendia J, Hawes P, Monaghan P, Cawthraw SA, Frankel G, Woodward MJ, Stevens MP. (2006) Role of intimin-tir interactions and the tir-cytoskeleton coupling protein in the colonization of calves and lambs by Escherichia coli O157:H7. Journal of Infect Immun , 74:758-64 . 
  • Abu-Median AB, van Diemen PM, Dziva F, Vlisidou I, Wallis TS, Stevens MP. (2006) Functional analysis of lymphostatin homologues in enterohaemorrhagic Escherichia coli. FEMS Microbiol Lett, 258:43-9 . 
  • Boyen F, Pasmans F, Donne E, Van Immerseel F, Morgan E, Adriaensen C, Hernalsteens JP, Wallis TS, Ducatelle R, Haesebrouck F. (2006) The fibronectin binding protein ShdA is not a prerequisite for long term faecal shedding of Salmonella typhimurium in pigs. Vet Microbiol, 115:284-90 . 
  • van Diemen PM, Dziva F, Stevens MP, Wallis TS. (2005) Identification of enterohemorrhagic Escherichia coli O26:H- genes required for intestinal colonization in calves. Infect Immun, 73:1735-43 . 
  • Marches O, Wiles S, Dziva F, La Ragione RM, Schuller S, Best A, Phillips AD, Hartland EL, Woodward MJ, Stevens MP, Frankel G (2005) Characterization of two non-locus of enterocyte effacement-encoded type III-translocated effectors, NleC and NleD, in attaching and effacing pathogens. Infect Immun, 73:8411-7. 
  • Stevens JM, Ulrich RL, Taylor LA, Wood MW, Deshazer D, Stevens MP, Galyov EE. (2005) Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant. Journal of Bacteriol , 187:7857-62 . 
  • Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, Hawes P, Monaghan P, Welch MD, Galyov EE (2005) Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei. JMol Microbiol, 56:40-53. 
  • Dahan S, Wiles S, La Ragione RM, Best A, Woodward MJ, Stevens MP, Shaw RK, Chong Y, Knutton S, Phillips A, Frankel G. (2005) EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics. Infect Immun, 73:679-86 
  • Upadhyay A, Williams C, Gill AC, Philippe DL, Davis K, Taylor LA, Stevens MP, Galyov EE, Bagby S. (2004) Biophysical characterization of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei. Biochim Biophys Acta, 1698:111-9. 
  • Zhang L, Chaudhuri RR, Constantinidou C, Hobman JL, Patel MD, Jones AC,Sarti D, Roe AJ, Vlisidou I, Shaw RK, Falciani F, Stevens MP, Gally DL, Knutton S, Frankel G, Penn CW, Pallen MJ. (2004) Regulators encoded in the Escherichia coli type III secretion system 2 gene cluster influence expression of genes within the locus for enterocyte effacement in enterohemorrhagic E. coli O157:H7. Infect Immun, 72:7282-93. 
  • Stevens MP, Haque A, Atkins T, Hill J, Wood MW, Easton A, Nelson M, Underwood-Fowler C, Titball RW, Bancroft GJ, Galyov EE. (2004) Attenuated virulence and protective efficacy of a Burkholderia pseudomallei bsa type III secretion mutant in murine models of melioidosis. Microbiology, 150:2669-76. 
  • Dziva F, van Diemen PM, Stevens MP, Smith AJ, Wallis TS. (2004) Identification of Escherichia coli O157 : H7 genes influencing colonization of the bovine gastrointestinal tract using signature-tagged mutagenesis. Microbiology, 150:3631-45 . 
  • Morgan E, Campbell JD, Rowe SC, Bispham J, Stevens MP, Bowen AJ, Barrow PA, Maskell DJ, Wallis TS. (2004) Identification of host-specific colonization factors of Salmonella enterica serovar Typhimurium. Mol Microbiol, 54:994-1010. 
  • Haque A, Bowe F, Fitzhenry RJ, Frankel G, Thomson M, Heuschkel R, Murch S, Stevens MP, Wallis TS, Phillips AD, Dougan G. (2004) Early interactions of Salmonella enterica serovar typhimurium with human small intestinal epithelial explants. Gut, 53:1424-30. 
  • Vlisidou I, Lyte M, van Diemen PM, Hawes P, Monaghan P, Wallis TS, Stevens MP. (2004) The neuroendocrine stress hormone norepinephrine augments Escherichia coli O157:H7-induced enteritis and adherence in a bovine ligated ileal loop model of infection. Infect Immun, 72:5446-51. 
  • Stevens MP, Roe AJ, Vlisidou I, van Diemen PM, La Ragione RM, Best A, Woodward MJ, Gally DL, Wallis TS. (2004) Mutation of toxB and a truncated version of the efa-1 gene in Escherichia coli O157:H7 influences the expression and secretion of locus of enterocyte effacement-encoded proteins but not intestinal colonization in calves or sheep. Infect Immun, 72:5402-11. 
  • Menge C, Stamm I, Van Diemen PM, Sopp P, Baljer G, Wallis TS, Stevens MP. (2004) Phenotypic and functional characterization of intraepithelial lymphocytes in a bovine ligated intestinal loop model of enterohaemorrhagic Escherichia coli infection. Journal of Med Microbiol, 53:573-9. 
  • Stevens MP, Friebel A, Taylor LA, Wood MW, Brown PJ, Hardt WD, Galyov EE. (2003) A Burkholderia pseudomallei type III secreted protein, BopE, facilitates bacterial invasion of epithelial cells and exhibits guanine nucleotide exchange factor activity. Journal of Bacteriol, 185:4992-6. 
  • Thomsen LE, Chadfield MS, Bispham J, Wallis TS, Olsen JE, Ingmer H. (2003) Reduced amounts of LPS affect both stress tolerance and virulence of Salmonella enterica serovar Dublin. FEMS Microbiol Lett, 228:225-31. 
  • Morris C, Tam CK, Wallis TS, Jones PW, Hackett J. (2003) Salmonella enterica serovar Dublin strains which are Vi antigen-positive use type IVB pili for bacterial self-association and human intestinal cell entry. Microb Pathog, 35:279-84. 
  • Fitzhenry RJ, Stevens MP, Jenkins C, Wallis TS, Heuschkel R, Murch S, Thomson M, Frankel G, Phillips AD (2003) Human intestinal tissue tropism of intimin epsilon O103 Escherichia coli. FEMS Microbiol Lett 218:311-6. 
  • Paulin SM, Watson PR, Benmore AR, Stevens MP, Jones PW, Villarreal-Ramos B,Wallis TS. (2002) Analysis of Salmonella enterica serotype-host specificity in calves: avirulence of S. enterica serotype gallinarum correlates with bacterial dissemination from mesenteric lymph nodes and persistence in vivo. Infect Immun, 70:6788-97.. 
  • Stevens MP, Wood MW, Taylor LA, Monaghan P, Hawes P, Jones PW, Wallis TS, Galyov EE. (2002) An Inv/Mxi-Spa-like type III protein secretion system in Burkholderia pseudomallei modulates intracellular behaviour of the pathogen. Mol Microbiol, 46:649-59.. 
  • Stevens MP, van Diemen PM, Frankel G, Phillips AD, Wallis TS. (2002) Efa1 influences colonization of the bovine intestine by shiga toxin-producing Escherichia coli serotypes O5 and O111. Infect Immun, 70:5158-66.. 
  • van Diemen PM, Kreukniet MB, Galina L, Bumstead N, Wallis TS. (2002) Characterisation of a resource population of pigs screened for resistance to salmonellosis. Vet Immunol Immunopathol, 88:183-96. . 
  • Sansone A, Watson PR, Wallis TS, Langford PR, Kroll JS. (2002) The role of two periplasmic copper- and zinc-cofactored superoxide dismutases in the virulence of Salmonella choleraesuis. Microbiology, 148:719-26. . 
  • Stevens MP, Marches O, Campbell J, Huter V, Frankel G, Phillips AD, Oswald E, Wallis TS. (2002) Intimin, tir, and shiga toxin 1 do not influence enteropathogenic responses to shiga toxin-producing Escherichia coli in bovine ligated intestinal loops. Infect Immun, 70:945-52. 
  • Schmitt CK, Ikeda JS, Darnell SC, Watson PR, Bispham J, Wallis TS, Weinstein DL, Metcalf ES, O'Brien AD. (2001) Absence of all components of the flagellar export and synthesis machinery differentially alters virulence of Salmonella enterica serovar Typhimurium in models of typhoid fever, survival in macrophages, tissue culture invasiveness, and calf enterocolitis. Infect Immun, 69:5619-25.
  • Monaghan P, Watson PR, Cook H, Scott L, Wallis TS, Robertson D. (2001) An improved method for preparing thick sections for immuno/histochemistry and confocal microscopy and its use to identify rare events. J Microsc, 203:223-6. . 
  • Uzzau S, Leori GS, Petruzzi V, Watson PR, Schianchi G, Bacciu D, Mazzarello V, Wallis TS, Rubino S. (2001) Salmonella enterica serovar-host specificity does not correlate with the magnitude of intestinal invasion in sheep. Infect Immun, 69:3092-9. . 
  • Ikeda JS, Schmitt CK, Darnell SC, Watson PR, Bispham J, Wallis TS, Weinstein DL, Metcalf ES, Adams P, O'Connor CD, O'Brien AD. (2001) Flagellar phase variation of Salmonella enterica serovar Typhimurium contributes to virulence in the murine typhoid infection model but does not influence Salmonella-induced enteropathogenesis. Infect Immun, 69:3021-30.. 
  • Bispham J, Tripathi BN, Watson PR, Wallis TS. (2001) Salmonella pathogenicity island 2 influences both systemic salmonellosis and Salmonella-induced enteritis in calves. Infect Immun, 69:367-77.. 
  • Phillips AD, Navabpour S, Hicks S, Dougan G, Wallis T, Frankel G. (2000) Enterohaemorrhagic Escherichia coli O157:H7 target Peyer's patches in humans and cause attaching/effacing lesions in both human and bovine intestine. Gut, 47:377-81.. 
  • Hartland EL, Daniell SJ, Delahay RM, Neves BC, Wallis T, Shaw RK, Hale C, Knutton S, Frankel G. (2000) The type III protein translocation system of enteropathogenic Escherichia coli involves EspA-EspB protein interactions. Mol Microbiol, 35:1483-92. . 
  • Wood MW, Jones MA, Watson PR, Siber AM, McCormick BA, Hedges S, Rosqvist R, Wallis TS, Galyov EE. (2000) The secreted effector protein of Salmonella dublin, SopA, is translocated into eukaryotic cells and influences the induction of enteritis. Cell Microbiol, 2:293-303.. 
  • Watson PR, Paulin SM, Jones PW, Wallis TS. (2000) Interaction of Salmonella serotypes with porcine macrophages in vitro does not correlate with virulence. Microbiology, 146:1639-49.. 
  • Watson PR, Benmore A, Khan SA, Jones PW, Maskell DJ, Wallis TS. (2000) Mutation of waaN reduces Salmonella enterica serovar Typhimurium-induced enteritis and net secretion of type III secretion system 1-dependent proteins. Infect Immun, 68:3768-71. . 
  • Watson PR, Gautier AV, Paulin SM, Bland AP, Jones PW, Wallis TS. (2000) Salmonella enterica serovars Typhimurium and Dublin can lyse macrophages by a mechanism distinct from apoptosis. Infect Immun 68:3744-7. . 
  • Bakshi CS, Singh VP, Wood MW, Jones PW, Wallis TS, Galyov EE. (2000) Identification of SopE2, a Salmonella secreted protein which is highly homologous to SopE and involved in bacterial invasion of epithelial cells. J Bacteriol 182:2341-4. . 
  • Schesser K, Dukuzumuremyi JM, Cilio C, Borg S, Wallis TS, Pettersson S, Galyov EE. (2000) The Salmonella YopJ-homologue AvrA does not possess YopJ-like activity. Microb Pathog 28:59-70.. 
  • Wallis TS, Wood M, Watson P, Paulin S, Jones M, Galyov E. (1999) Sips, Sops, and SPIs but not stn influence Salmonella enteropathogenesis. Adv Exp Med Biol 473:275-80.. 
  • Bolton AJ, Osborne MP, Wallis TS, Stephen J. (1999) Interaction of Salmonella choleraesuis, Salmonella dublin and Salmonella typhimurium with porcine and bovine terminal ileum in vivo. Microbiology 145:2431-41.. 
  • Bolton AJ, Martin GD, Osborne MP, Wallis TS, Stephen J. (1999) Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro. J Med Microbiol 48:801-10. . 
  • Watson PR, Paulin SM, Bland AP, Libby SJ, Jones PW, Wallis TS. (1999) Differential regulation of enteric and systemic salmonellosis by slyA. Infect Immun 67:4950-4. . 
  • Ahmer BM, van Reeuwijk J, Watson PR, Wallis TS, Heffron F. (1999) Salmonella SirA is a global regulator of genes mediating enteropathogenesis. Mol Microbiol 31:971-82.. 
  • Norris FA, Wilson MP, Wallis TS, Galyov EE, Majerus PW. (1998) SopB, a protein required for virulence of Salmonella dublin, is an inositol phosphate phosphatase. Proc Natl Acad Sci U S A 95:14057-9. . 
  • Jones MA, Wood MW, Mullan PB, Watson PR, Wallis TS, Galyov EE. (1998) Secreted effector proteins of Salmonella dublin act in concert to induce enteritis. Infect Immun 66:5799-804.. 
  • Wood MW, Jones MA, Watson PR, Hedges S, Wallis TS, Galyov EE. (1998) Identification of a pathogenicity island required for Salmonella enteropathogenicity. Mol Microbiol 29:883-91.. 
  • Watson PR, Galyov EE, Paulin SM, Jones PW, Wallis TS. (1998) Mutation of invH, but not stn, reduces Salmonella-induced enteritis in cattle. Infect Immun 66:1432-8.. 
  • Galyov EE, Wood MW, Rosqvist R, Mullan PB, Watson PR, Hedges S, Wallis TS. (1997) A secreted effector protein of Salmonella dublin is translocated into eukaryotic cells and mediates inflammation and fluid secretion in infected ileal mucosa. Mol Microbiol 25:903-12.. 
  • Frost AJ, Bland AP, Wallis TS. (1997) The early dynamic response of the calf ileal epithelium to Salmonella typhimurium. Vet Pathol 34:369-86. . 
  • Farrant JL, Sansone A, Canvin JR, Pallen MJ, Langford PR, Wallis TS, Dougan G, Kroll JS. (1997) Bacterial copper- and zinc-cofactored superoxide dismutase contributes to the pathogenesis of systemic salmonellosis. Mol Microbiol 25:785-96. . 
  • Guilloteau LA, Wallis TS, Gautier AV, MacIntyre S, Platt DJ, Lax AJ. (1996) The Salmonella virulence plasmid enhances Salmonella-induced lysis of macrophages and influences inflammatory responses. Infect Immun 64:3385-93. . 
  • Guilloteau LA, Lax AJ, MacIntyre S, Wallis TS. (1996) The Salmonella dublin virulence plasmid does not modulate early T-cell responses in mice. Infect Immun 64:222-9.. 
  • Wallis TS, Paulin SM, Plested JS, Watson PR, Jones PW. (1995) The Salmonella dublin virulence plasmid mediates systemic but not enteric phases of salmonellosis in cattle. Infect Immun 63:2755-61. . 
  • Watson PR, Paulin SM, Bland AP, Jones PW, Wallis TS. (1995) Characterization of intestinal invasion by Salmonella typhimurium and Salmonella dublin and effect of a mutation in the invH gene. Infect Immun 63:2743-54. . 

Review articles & book chapters

  • Stevens MP. (2007) Enterohaemorrhagic Escherichia coli virulence genes- progress toward their identification and prospects for control in food-producing animals. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 1, No. 068. . 
  • Stevens JM, Galyov EE, Stevens MP. (2006) Actin-dependent movement of bacterial pathogens. Nat Rev Microbiol 4:91-101. 
  • Morgan E. (2006) Salmonella pathogenicity islands. In Salmonella pathogenesis, molecular typing and epidemiology, Eds D.J. Maskell, M. Rhen. Horizon Scientific Press, Norwich, UK.
  • Stevens MP, Wallis TS. (2005) Adhesins of enterohaemorrhagic Escherichia coli. In EcoSal-Escherichia coli and Salmonella: cellular and molecular biology. Module 8.3.3.2. Ed. J. Kaper [Online]. ASM Press, Washington, D.C. 
  • Wallis TS, & Barrow PA. (2005) Salmonella epidemiology and pathogenesis in food-producing animals. In EcoSal-Escherichia coli and Salmonella: cellular and molecular biology. Module 8.6.2.1. Ed. R. Curtiss . [Online.] . ASM Press, Washington, D.C. 
  • Stevens MP, Galyov EE (2004) Exploitation of host cells by Burkholderia pseudomallei. Int J Med Microbiol 293:549-55.  
  • Wallis TS. (2004) Vaccination against salmonella, enterohaemorrhagic E. coli and Campylobacter in food-producing animals. Dev Biol (Basel) 119:343-50. . 
  • Stevens MP, van Diemen PM, Dziva F, Jones PW, Wallis TS. (2002) Options for the control of enterohaemorrhagic Escherichia coli in ruminants. Microbiology 148:3767-78.. 
  • Wallis TS, Galyov EE. (2000) Molecular basis of Salmonella-induced enteritis. Mol Microbiol 36:997-1005. . 
  • Lax AJ, Barrow PA, Jones PW, Wallis TS. (1995) Current perspectives in salmonellosis. Br Vet J 151:351-77. .